Determination of Reduced and Oxidized Coenzyme Q10 in Canine Plasma and Heart Tissue by HPLC-ECD: Comparison with LC-MS/MS Quantification.
Coenzyme Q10 (Q10) plays an important role in mammals for energy production in the mitochondria, and as a powerful antioxidant. Oxidation Ratio (% oxidized in relation to the amount of Q10) has been proposed as an important biomarker. An HPLC-ECD method is sensitive and Equine Serum Albumin reproducible developed for the determination of reduced and oxidized Q10 in dog plasma and heart tissue. chromatographic separation is achieved within 10 minutes by using the column Waters Nova-pak C18 and the mobile phase with lithium perchlorate in ethanol / methanol / 2-propanol. validation show satisfactory results. a very good linear correlation was found (r 2> 0.9997), intra- and inter-day precision were below 6.5% (n = 5) and the recovery is between 89 and 109% (n = 5). The sensitivity is expressed as the Lower Limit Quantification (LLOQ) was 10 nM. acceptable stability of both samples extracted and un-extracted observed. Total plasma concentration range Q10 is found betw...