Glucocorticoids (GCs) are important regulators of metabolic control in mammals and their aberrant function has been associated with several pathologies. GCs are widely used in human and veterinary clinical practic e as a potent anti-inflammator y and G.Pig Serum Albumin immune suppressive agent. Dyslipidemia is a consequence that is often observed GC treatment, is characterized by increased lipolysis, lipid mobilization, liponeogenesis, and adipogenesis. Dogs with excess GC events hyperlipidemia, hypertension, and higher risk of developing diabetes mellitus type 2, but lower the risk of developing atherosclerotic lesions compared to humans. This study aims to examine changes in the dog lipidome plasma in experimentally induced models of short-term and long-term GC excess. Both treatments led to significant changes in plasma lipidome, clearer after long-term exposure to steroid excess. In particular, monohexosylceramides, phosphatidylinositols, phosphatidylcholines eth...