Changes in the Canine Plasma Lipidome after Short- and Long-Term Excess Glucocorticoid Exposure.

Glucocorticoids (GCs) are important regulators of metabolic control in mammals and their aberrant function has been associated with several pathologies. GCs are widely used in human and veterinary clinical practice as a potent anti-inflammatory and G.Pig Serum Albumin immune suppressive agent. Dyslipidemia is a consequence that is often observed GC treatment, is characterized by increased lipolysis, lipid mobilization, liponeogenesis, and adipogenesis.

Dogs with excess GC events hyperlipidemia, hypertension, and higher risk of developing diabetes mellitus type 2, but lower the risk of developing atherosclerotic lesions compared to humans. This study aims to examine changes in the dog lipidome plasma in experimentally induced models of short-term and long-term GC excess. Both treatments led to significant changes in plasma lipidome, clearer after long-term exposure to steroid excess.

In particular, monohexosylceramides, phosphatidylinositols, phosphatidylcholines ether, acyl phosphatidylcholines, triglycerides and sphingosine 1-phosphate showed a significant change. Now the study highlights the hitherto unknown effect of GCS on lipid metabolism, which will be important in further explanation of the role and function of GCS as a drug and the metabolic and cardiovascular diseases.

Changes in the Canine Plasma Lipidome after Short- and Long-Term Excess Glucocorticoid Exposure.

In vitro assessment of the long-term stability of the inhibitory effect of plasma of dogs and cats on MMP 2 and 9.

To evaluate the effect of increasing the storage time on the inhibitory effect of plasma of dogs and cats in the matrix metalloproteinase (MMP) 2 and 9 in vitro.Matrix metalloproteinase 2 and 9 activity in the presence of a dog plasma stored for 57, 155, 222, 316, 367, and 438 days and plasma cats kept for 17, 198, 565, and 954 days was tested using a commercially available colorimetric assay kit.

For dog plasma, the activity of MMP 2 for the Goat Plasma older samples did not differ significantly from the 57- day samples (P = 0, 2025-.9033). Two samples of dogs has significantly lowered the activity of MMP-9 from the sample 57 days (367 days: P = 0.0099, 438 day: P = 0.0348, P = 0.0778 to 0.9928 else).

For plasma cats, storage time did not significantly affect inhibition of MMP 2 and MMP 9 activity (ANOVA, P = 0.2688 and P = 0.2404, respectively) .Increasing storage time did not significantly decrease the activity of plasma MMP inhibiting 2 and 9 for up to 14 months in dogs and 31 months in cats.

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Determination of Reduced and Oxidized Coenzyme Q10 in Canine Plasma and Heart Tissue by HPLC-ECD: Comparison with LC-MS/MS Quantification.

Coenzyme Q10 (Q10) plays an important role in mammals for energy production in the mitochondria, and as a powerful antioxidant. Oxidation Ratio (% oxidized in relation to the amount of Q10) has been proposed as an important biomarker. An HPLC-ECD method is sensitive and Equine Serum Albumin reproducible developed for the determination of reduced and oxidized Q10 in dog plasma and heart tissue. chromatographic separation is achieved within 10 minutes by using the column Waters Nova-pak C18 and the mobile phase with lithium perchlorate in ethanol / methanol / 2-propanol. validation show satisfactory results. a very good linear correlation was found (r 2> 0.9997), intra- and inter-day precision were below 6.5% (n = 5) and the recovery is between 89 and 109% (n = 5). The sensitivity is expressed as the Lower Limit Quantification (LLOQ) was 10 nM. acceptable stability of both samples extracted and un-extracted observed. Total plasma concentration range Q10 is found betw...

A placebo-controlled study comparing the efficacy of intra-articular injections of hyaluronic acid and a novel hyaluronic acid-platelet-rich plasma conjugate in a canine model of osteoarthritis.

The purpose of this study was to assess the efficacy of intra-articular injection of hyaluronic acid (HA) and a novel, where the conjugate of HA with fibrinogen autologous be platelet rich plasma (HA-PRP) in a canine model of osteoarthritis (OA) METHOD : Twelve Donkey Serum beagle dogs underwent unilateral resection of the cranial cruciate ligament (CrCl) of the joint crippling. clinical signs and radiographic OA was confirmed in all dogs 8 weeks after CrCl resection and before treatment. The dogs were randomized into three groups: saline (n = 4), HA (n = 4), and HA-PRP (n = 4). Each dog receives intra-articular injections of each substance into the affected joint in a pre-determined point in time. The dogs were assessed for adverse events during 3 days after each injection and for lameness, pain, range of motion, kinetics, and radiographic OA severity before treatment and 3 months after injection. OA severity as determined by radiographic examination did not differ s...

Assessment of the Efficacy of Platelet-Rich Plasma in the Treatment of Traumatic Canine Fractures.

Role of platelet rich plasma (PRP) to promote fracture healing has not been stated clearly. The aim of this prospective clinical study was to evaluate the effectiveness of plasma rich in growth factors (PRGF, derivate PRP) in the treatment of bone fracture occurs naturally in dogs. With the goal, sixty-five-dog with Goat Serum a radius / ulna or tibia / fracture of the fibula were randomly divided into two groups (PRGF and saline solution (SS) group) and examined at days 0, 7, 14, 21, 28, 35, 42, 49, 56, 60, 63, 70, 120, and 180. All fractures treated with external skeletal fixation, and pain is controlled with carprofen. Healing was evaluated by physical examination, limb function, radiographic, and by Likert-type questionnaires owner satisfaction. A more rapid healing of fractures observed in PRGF group, with a statistically significant difference against the SS. Swelling at the site of fracture was significantly greater at 14 and 28 days in animals injected with t...

Canine Plasma

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