Skip to main content

Platelet-rich plasma affects the proliferation of canine bone marrow-derived mesenchymal stromal cells in vitro.

reported the efficacy of platelet rich plasma (PRP) in regenerative medicine contradictory. We validated the PRP effect on cell proliferation dog bone marrow-derived multipotent mesenchymal stromal (K9BMMSCs) in vitro. PRP is extracted from the Feline Serum blood of six dogs with osteoarthritis. K9BMMSCs established from the bone marrow and is characterized for CD90 and CD19 expression by immunocytochemistry. PRP concentration influence on the survival of autologous K9BMMSCs suitable validated using MTS assay.

Positive negative CD90 and CD19 expression was confirmed MSC origin. PRP at concentrations volume / volume increased 40%, while PRP at 80 and 100% v / v concentration tested suppressed viability K9BMMSCs.PRP concentration play an important role in the survival of K9BMMSCs, which can affect tissue repair in vivo.
Platelet-rich plasma affects the proliferation of canine bone marrow-derived mesenchymal stromal cells in vitro.

The ability to distinguish four plasma biomarkers in canine hip dysplasia.


The accumulation of breakdown products of cartilage in the body fluids have been studied extensively to assess the accuracy of biomarker molecules from the perspective of diagnostic, prognostic, and therapeutic. However, to the authors' knowledge, there is a lack of information about the model of spontaneous hip osteoarthritis and the ability to distinguish the collagen, noncollagen and inflammatory biomarkers.

We aim to assess the accuracy of four plasma biomarkers that could distinguish between healthy dogs and dogs with hip dysplasia.Twenty-four the dogs were used in this study are institutionally approved (12 in mild to severe hip dysplasia group; 12 in the control group). Plasma concentrations of biomarkers compared. The ability of each marker to distinguish control of diseased dogs were assessed using independent t-test, logistic regression, and receiving operating characteristic (ROC) analysis.Three biomarkers differed significantly between the two groups. 

Markers of collagen type II procollagen propeptide (PIICP) is useful in distinguishing between control and diseased dog with the best combination of sensitivity and specificity. Fourth biomarker showed high area under the curve results (AUC) values.The showed that plasma biomarkers can be used as a screening tool for canine hip dysplasia. Although Feline Serum Albumin the cutoff values ​​and diagnostic capabilities of biomarkers used in this study showed promising results, the sources of individual variability should be addressed. future studies with larger groups of dogs are required to correlate plasma levels in serum and synovial fluid during clinical disease.
Labels:

Comments

Post a Comment

Popular posts from this blog

A placebo-controlled study comparing the efficacy of intra-articular injections of hyaluronic acid and a novel hyaluronic acid-platelet-rich plasma conjugate in a canine model of osteoarthritis.

The purpose of this study was to assess the efficacy of intra-articular injection of hyaluronic acid (HA) and a novel, where the conjugate of HA with fibrinogen autologous be platelet rich plasma (HA-PRP) in a canine model of osteoarthritis (OA) METHOD : Twelve  Donkey Serum beagle dogs underwent unilateral resection of the cranial cruciate ligament (CrCl) of the joint crippling. clinical signs and radiographic OA was confirmed in all dogs 8 weeks after CrCl resection and before treatment.  The dogs were randomized into three groups: saline (n = 4), HA (n = 4), and HA-PRP (n = 4). Each dog receives intra-articular injections of each substance into the affected joint in a pre-determined point in time. The dogs were assessed for adverse events during 3 days after each injection and for lameness, pain, range of motion, kinetics, and radiographic OA severity before treatment and 3 months after injection.  OA severity as determined by radiographic examination did not differ s...
Post a Comment
Read more

Metabolomic profiling to identify effects of dietary calcium reveal the influence of the individual and postprandial dynamics on the canine plasma metabolome.

Short-term studies have highlighted the phenomenon meal in the regulation of Ca salary increase concerns about the absorption of Ca in dogs that can make an impact on the commercial diet close to the maximum recommended levels. A recent  G.Pig Plasma study to determine the response of the dog ate one of two diets differed in Ca diet over 40 weeks did not find evidence that shows concerns in the various hypotheses of biological parameters will be affected by Ca. An unexpected consequence of food Ca could occur and metabolic profile is considered data-driven approach that is suitable to identify the effects of Ca.  The goal is to compare the diet fasting plasma metabolome (sampled at intervals of 8 weeks more than 40 weeks) dog ate one of two diets, close to the minimum and the maximum recommended level of Ca. Comparison with diet control diet was also investigated in the course of time postprandial (1-4 h) following acute (1 d) and long term (24 weeks) fed a diet test. Compar...
Post a Comment
Read more