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Platelet-rich plasma affects the proliferation of canine bone marrow-derived mesenchymal stromal cells in vitro.

reported the efficacy of platelet rich plasma (PRP) in regenerative medicine contradictory. We validated the PRP effect on cell proliferation dog bone marrow-derived multipotent mesenchymal stromal (K9BMMSCs) in vitro. PRP is extracted from the Feline Serum blood of six dogs with osteoarthritis. K9BMMSCs established from the bone marrow and is characterized for CD90 and CD19 expression by immunocytochemistry. PRP concentration influence on the survival of autologous K9BMMSCs suitable validated using MTS assay.

Positive negative CD90 and CD19 expression was confirmed MSC origin. PRP at concentrations volume / volume increased 40%, while PRP at 80 and 100% v / v concentration tested suppressed viability K9BMMSCs.PRP concentration play an important role in the survival of K9BMMSCs, which can affect tissue repair in vivo.
Platelet-rich plasma affects the proliferation of canine bone marrow-derived mesenchymal stromal cells in vitro.

The ability to distinguish four plasma biomarkers in canine hip dysplasia.


The accumulation of breakdown products of cartilage in the body fluids have been studied extensively to assess the accuracy of biomarker molecules from the perspective of diagnostic, prognostic, and therapeutic. However, to the authors' knowledge, there is a lack of information about the model of spontaneous hip osteoarthritis and the ability to distinguish the collagen, noncollagen and inflammatory biomarkers.

We aim to assess the accuracy of four plasma biomarkers that could distinguish between healthy dogs and dogs with hip dysplasia.Twenty-four the dogs were used in this study are institutionally approved (12 in mild to severe hip dysplasia group; 12 in the control group). Plasma concentrations of biomarkers compared. The ability of each marker to distinguish control of diseased dogs were assessed using independent t-test, logistic regression, and receiving operating characteristic (ROC) analysis.Three biomarkers differed significantly between the two groups. 

Markers of collagen type II procollagen propeptide (PIICP) is useful in distinguishing between control and diseased dog with the best combination of sensitivity and specificity. Fourth biomarker showed high area under the curve results (AUC) values.The showed that plasma biomarkers can be used as a screening tool for canine hip dysplasia. Although Feline Serum Albumin the cutoff values ​​and diagnostic capabilities of biomarkers used in this study showed promising results, the sources of individual variability should be addressed. future studies with larger groups of dogs are required to correlate plasma levels in serum and synovial fluid during clinical disease.
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