Canine Plasma

Glucocorticoids (GCs) are important regulators of metabolic control in mammals and their aberrant function has been associated with several pathologies. GCs are widely used in human and veterinary clinical practic e as a potent anti-inflammator y and  G.Pig Serum Albumin immune suppressive agent. Dyslipidemia is a consequence that is often observed GC treatment, is characterized by increased lipolysis, lipid mobilization, liponeogenesis, and adipogenesis.  Dogs with excess GC events hyperlipidemia, hypertension, and higher risk of developing diabetes mellitus type 2, but lower the risk of developing atherosclerotic lesions compared to humans. This study aims to examine changes in the dog lipidome plasma in experimentally induced models of short-term and long-term GC excess. Both treatments led to significant changes in plasma lipidome, clearer after long-term exposure to steroid excess.  In particular, monohexosylceramides, phosphatidylinositols, phosphatidylcholines eth...

Short-term studies have highlighted the phenomenon meal in the regulation of Ca salary increase concerns about the absorption of Ca in dogs that can make an impact on the commercial diet close to the maximum recommended levels. A recent  G.Pig Plasma study to determine the response of the dog ate one of two diets differed in Ca diet over 40 weeks did not find evidence that shows concerns in the various hypotheses of biological parameters will be affected by Ca. An unexpected consequence of food Ca could occur and metabolic profile is considered data-driven approach that is suitable to identify the effects of Ca.  The goal is to compare the diet fasting plasma metabolome (sampled at intervals of 8 weeks more than 40 weeks) dog ate one of two diets, close to the minimum and the maximum recommended level of Ca. Comparison with diet control diet was also investigated in the course of time postprandial (1-4 h) following acute (1 d) and long term (24 weeks) fed a diet test. Compar...

reported the efficacy of platelet rich plasma (PRP) in regenerative medicine contradictory. We validated the PRP effect on cell proliferation dog bone marrow-derived multipotent mesenchymal stromal (K9BMMSCs) in vitro. PRP is extracted from the  Feline Serum blood of six dogs with osteoarthritis. K9BMMSCs established from the bone marrow and is characterized for CD90 and CD19 expression by immunocytochemistry. PRP concentration influence on the survival of autologous K9BMMSCs suitable validated using MTS assay. Positive negative CD90 and CD19 expression was confirmed MSC origin. PRP at concentrations volume / volume increased 40%, while PRP at 80 and 100% v / v concentration tested suppressed viability K9BMMSCs.PRP concentration play an important role in the survival of K9BMMSCs, which can affect tissue repair in vivo. The ability to distinguish four plasma biomarkers in canine hip dysplasia. The accumulation of breakdown products of cartilage in the body fluids have been studied...

Coenzyme Q10 (Q10) plays an important role in mammals for energy production in the mitochondria, and as a powerful antioxidant. Oxidation Ratio (% oxidized in relation to the amount of Q10) has been proposed as an important biomarker.  An HPLC-ECD method is sensitive and  Equine Serum Albumin reproducible developed for the determination of reduced and oxidized Q10 in dog plasma and heart tissue. chromatographic separation is achieved within 10 minutes by using the column Waters Nova-pak C18 and the mobile phase with lithium perchlorate in ethanol / methanol / 2-propanol. validation show satisfactory results. a very good linear correlation was found (r 2> 0.9997), intra- and inter-day precision were below 6.5% (n = 5) and the recovery is between 89 and 109% (n = 5).  The sensitivity is expressed as the Lower Limit Quantification (LLOQ) was 10 nM. acceptable stability of both samples extracted and un-extracted observed. Total plasma concentration range Q10 is found betw...

The purpose of this study was to assess the efficacy of intra-articular injection of hyaluronic acid (HA) and a novel, where the conjugate of HA with fibrinogen autologous be platelet rich plasma (HA-PRP) in a canine model of osteoarthritis (OA) METHOD : Twelve  Donkey Serum beagle dogs underwent unilateral resection of the cranial cruciate ligament (CrCl) of the joint crippling. clinical signs and radiographic OA was confirmed in all dogs 8 weeks after CrCl resection and before treatment.  The dogs were randomized into three groups: saline (n = 4), HA (n = 4), and HA-PRP (n = 4). Each dog receives intra-articular injections of each substance into the affected joint in a pre-determined point in time. The dogs were assessed for adverse events during 3 days after each injection and for lameness, pain, range of motion, kinetics, and radiographic OA severity before treatment and 3 months after injection.  OA severity as determined by radiographic examination did not differ s...